Roger W. Hendrix
Professor,
Department of Biological Sciences
A340A Langley Hall
412-624-4674,
4870(fax)
rhx+@pitt.edu
http://www.pitt.edu/~biohome/Dept/Frame/Faculty/hendrix.htm
Roger Hendrix and his research group study the dsDNA bacteriophages
(bacterial viruses) to investigate the mechanisms viruses use to
assemble themselves accurately from several hundred protein
subunits and a DNA molecule. Bacteriophages HK97 and HK022,
illustrated in the figure, complete their maturation ‘dance’ by
forming 420 covalent crosslinks among the 420 protein subunits
of the capsid. This results in ‘viral chainmail’, a structure in
which covalent rings of protein are topologically linked into a
continuous fabric. These studies teach us what viruses have
learned about nanofabrication over the past 3-4 billion years of
evolution and also provide a scaffold for attaching molecules of
interest.

|
A negatively stained electron micrograph
of the viruses. Colors on the heads illustrate the
connectivity of the chainmail crosslinks. |